JCRB1866 BT-C

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB1866 Cell Name   BT-C
Profile   bovine trophoblastic cell line Other Name   
Animal   bovine Strain   
Genus   Bos Species   taurus
Sex    Age   
Identity   not done Tissue for Primary Cancer   
Case history    Metastasis   
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   
Classify   normal Established by   Hashizume K., Takahashi T.
Registered by   Keiichiro Kizaki Regulation for Distribution   
Comment   BMP4 concentration at cell establishment: 10 ng/mL Year   
Medium   DMEM/F12 medium + 10% FBS (Culture in 20% FBS when thawing), Use collagen-coated dish or flask (Cellmatrix Type I-C, Nitta Gelatin, Osaka,Japan) Methods for Passages   Pipetting dissociation and dilution.
Cell Number on Passage    Race   
CO2 Conc.   5% Tissue Sampling   blastcyst
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Reference
Pubmed id:21862694Bone morphogenetic protein 4 accelerates the establishment of bovine trophoblastic cell lines.
Suzuki Y,Koshi K,Imai K,Takahashi T,Kizaki K,Hashizume K
Reproduction. 2011 Nov;142(5):733-43

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LOT Information

Cell No.   JCRB1866 Cell Name   BT-C
LOT No.   09022021 Lot Specification   distribution
Medium   DMEM/ F12(1/1) medium(SIGMA,#D8062-500ML) with 10%(20%) heat inactivated fetal bovine serum(japan-bioserum,NZP 001,Lot CNGGR-100) Temperature   37 C
Cell Density at Seeding   NT Methods for Passages   pipetting dissociation and dilution.（split ratio = 1/2-1/4) (collagen type I coated Flask(CORNING,NCO430639))
Doubling Time   NT Cell Number in Vial (cells/1ml)   uncountable
Viability at cell freezing (%)   uncountable Antibiotics Used   free
Passage Number   p69 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   BAMBANKER(LYMPHOTEC Inc.,CS-02-001,NIPPON Genetics Co., LTD) CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   NT
Additional information   Type of ampoules is plastic. We recommend to culture initially with 20 % FBS.
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