JCRB1871 Akata Δgp85

Cell information

Important Notice(s)

JCRB1871 Akata Δgp85のEBV潜伏感染について

Cell type:virus production cells (View Pricing Information)

JCRB No.   JCRB1871 Cell Name   Akata Δgp85
Profile   EBV-encoded glycoprotein 85 (gp 85) knockout EBV was reinfected into Akata- cell line. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   F Age   4-year-old
Identity   not done Tissue for Primary Cancer   blood
Case history   Burkitt’s lymphoma Metastasis   
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   lymphocyte-like Character   
Classify   transformed Established by   Kenzo Takada
Registered by   Kenzo Takada Regulation for Distribution   
Comment    Year   2019
Medium   RPMI1640 with 10% FBSand G418. (Lot check of FBS is important) Methods for Passages   dilution
Cell Number on Passage    Race   
CO2 Conc.   5% Tissue Sampling   blood
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV + HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -

Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_B6R7
Pubmed id:11021994Epstein-Barr virus lacking glycoprotein gp85 cannot infect B cells and epithelial cells.
Oda T,Imai S,Chiba S,Takada K
Virology. 2000 Oct 10;276(1):52-8


LOT Information

Cell No.   JCRB1871 Cell Name   Akata Δgp85
LOT No.   10122021 Lot Specification   distribution
Medium   RPMI1640(GIBCO Cat#11875-093) with 10% heat inactivated fetal bovine serum(SIGMA,Cat#172012,Lot#12J396) and G418(700 ug/ml) Temperature   37 C
Cell Density at Seeding   1.0-2.5x10^5 cells/ml Methods for Passages   dilution
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.7x10^6
Viability at cell freezing (%)   92.50 Antibiotics Used   G418
Passage Number   p4* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:11,12
Adhesion   No Exoteric Gene   NT
Medium for Freezing   BAMBANKER(LYMPHOTEC Inc.,CS-02-001,NIPPON Genetics Co., LTD) CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   85.30
Additional information