JCRB1872 OCUT-6

Cell information

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB1872 Cell Name   OCUT-6
Profile   human anaplastic thyroid cancer cell line Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   F Age   68-year-old
Identity   available Tissue for Primary Cancer   thyroid
Case history    Metastasis   Y
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   floating Character   
Classify   tumor Established by   Onoda N.
Registered by   Osaka City Univ. Graduate School of Medicine Regulation for Distribution   
Comment    Year   
Medium   DMEM high glucose + 10% FBS Methods for Passages   0.05% Trypsin - 0.02% EDTA
Cell Number on Passage    Race   Japanese
CO2 Conc.   5% Tissue Sampling   thyroid
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus not tested
CMV - parvoB19 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
EBV - HBV -
HHV6 - HTLV-1 -
HHV7 - HTLV-2 -
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Reference
Pubmed id:24357248Establishment, characterization and comparison of seven authentic anaplastic thyroid cancer cell lines retaining clinical features of the original tumors.
Onoda N,Nakamura M,Aomatsu N,Noda S,Kashiwagi S,Hirakawa K
World J Surg. 2014 Mar;38(3):688-95

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LOT Information

Cell No.   JCRB1872 Cell Name   OCUT-6
LOT No.   10222021 Lot Specification   distribution
Medium   DMEM (high glucose:GIBCO Cat#11995065) with 10% heat inactivated fetal bovine serum (SIGMA, Cat#172012, Lot#12J396) Temperature   37 C
Cell Density at Seeding   1.3-2x10^4 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.05%trypsin (GIBCO) and 0.02% EDTA. (split ratio=1/6-1/2)
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.70x10^6
Viability at cell freezing (%)   95.3 Antibiotics Used   free
Passage Number   p10* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:10,13
D13S317:11
D7S820:10,11
D16S539:9,10
VWA:17
TH01:6
AM:X
TPOX:11
CSF1PO:11
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   BAMBANKER (LYMPHOTEC Inc., CS-02-001, NIPPON Genetics Co., LTD) CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   94.1
Additional information   
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