JCRB1873 HSC-44PE

Cell information

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB1873 Cell Name   HSC-44PE
Profile   Human signet ring cell gastric carcinoma cell line. Established from tumor cells in pleural effusion. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex    F Age   28-year-old
Identity   available Tissue for Primary Cancer   stomach
Case history   scirrhous stomach cancer Metastasis   
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   epithelial-like and round shape Character   HSC-45 established from same patient. Partially adherent cells.
Classify   tumor Established by   Yanagihara Kazuyoshi
Registered by   Yuki Iino Regulation for Distribution   
Comment    Year   
Medium   RPMI1640 medium + 10% FBS Methods for Passages   0.05% Trypsin-0.02% EDTA
Cell Number on Passage    Race   Japanese
CO2 Conc.   5% Tissue Sampling   pleural effusion
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Reference
Pubmed id:15245593Establishment of two cell lines from human gastric scirrhous carcinoma that possess the potential to metastasize spontaneously in nude mice.
Yanagihara K,Tanaka H,Takigahira M,Ino Y,Yamaguchi Y,Toge T,Sugano K,Hirohashi S
Cancer Sci. 2004 Jul;95(7):575-82

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LOT Information

Cell No.   JCRB1873 Cell Name   HSC-44PE
LOT No.   10152021 Lot Specification   distribution
Medium   RPMI1640 medium (GIBCO:Cat No.11875) with 10% heat inactivated fetal bovine serum (SIGMA, Cat#172012-500ML, Lot#12J396) Temperature   37 C
Cell Density at Seeding   1.14x10^4 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.25%trypsin (GIBCO) and 0.02% EDTA. (Patially adherent cells)
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.64x10^6
Viability at cell freezing (%)   94 Antibiotics Used   free
Passage Number   p3* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:10
D13S317:8
D7S820:11
D16S539:13
VWA:17
TH01:8,9
AM:X
TPOX:8
CSF1PO:11,12
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   BAMBANKER (LYMPHOTEC Inc., CS-02-001, NIPPON Genetics Co., LTD) CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   97
Additional information   
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