JCRB1875 BT-1S

Cell information

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BT細胞系の培養について

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB1875 Cell Name   BT-1S
Profile   bovine trophoblastic cell line Other Name   
Animal   bovine Strain   
Genus   Bos Species   taurus
Sex    Age   
Identity   not done Tissue for Primary Cancer   
Case history    Metastasis   
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   
Classify   normal Established by   Hashizume K., Takahashi T., Shimada A.
Registered by   Keiichiro Kizaki Regulation for Distribution   
Comment    Year   
Medium   DMEM/F12 medium + 10% FBS (Culture in 20% FBS when thawing), Use collagen-coated dish or flask (Cellmatrix Type I-C, Nitta Gelatin, Osaka,Japan) Methods for Passages   Pipetting dissociation and dilution.
Cell Number on Passage    Race   
CO2 Conc.   5% Tissue Sampling   blastcyst
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Reference
Pubmed id:16251743Bovine trophoblast cell culture systems: a technique to culture bovine trophoblast cells without feeder cells.
Hashizume K,Shimada A,Nakano H,Takahashi T
Methods Mol Med. 2006;121():179-88

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LOT Information

Cell No.   JCRB1875 Cell Name   BT-1S
LOT No.   12092021 Lot Specification   distribution
Medium   DMEM/ F12(1/1) medium (SIGMA, #D8062-500ML) with 10% (20%) heat inactivated fetal bovine serum (japan-bioserum,NZP 001, Lot CNGGR-100) Temperature   37 C
Cell Density at Seeding   uncountable Methods for Passages   Pipetting dissociation and dilution (split ratio=1/2-1/4). (Collagen type I coated flask (CORNING, NCO430639)
Doubling Time   NT Cell Number in Vial (cells/1ml)   uncountable
Viability at cell freezing (%)   uncountable Antibiotics Used   free
Passage Number   p216 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   BAMBANKER (LYMPHOTEC Inc., CS-02-001, NIPPON Genetics Co., LTD) CO2 Conc.   5%
Viability immediately after thawing (%)   uncountable Additional information   We recommend to culture initially with 20% heat inactivated fetal bovine serum.
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