JCRB1882 Sui65

Cell information

Important Notice(s)

Sui細胞株に関する遺伝的性質について(About the genetic properties of Sui cell lines)

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB1882 Cell Name   Sui65
Profile   Pancreatic adenocarcinoma. Cell line established by forming xenograft tumor (PDX) from peritoneal metastasis. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   F Age   63-year-old
Identity   available Tissue for Primary Cancer   pancreas
Case history   Pancreatic tubular adenocarcinoma. Derived from metastatic site: Peritoneum. Metastasis   Y
Tissue Metastasized   Peritoneum Genetics   Mutation of CDKN2A, KRAS, TP53, and SMAD4. See Important notice for details.
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   Doubling time: 48.6 hours
Classify   tumor Established by   Yanagihara Kazuyoshi
Registered by   Yuki Iino Regulation for Distribution   
Comment    Year   
Medium   PMI1640 medium + 10% FBS Methods for Passages   0.05% Trypsin- 0.02% EDTA
Cell Number on Passage   split ratio = 1/5 Race   
CO2 Conc.   5% Tissue Sampling   Peritoneum
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -

Pubmed id:35272288A Comparative Study of Patient-Derived Tumor Models of Pancreatic Ductal Adenocarcinoma Involving Orthotopic Implantation.
Yanagihara K,Iino Y,Yokozaki H,Kubo T,Oda T,Kubo T,Komatsu M,Sasaki H,Ichikawa H,Kuwata T,Seyama T,Ochiai A
Pathobiology. 2022 Mar 10;():1-11
Pubmed id:18691340Establishment and molecular profiling of a novel human pancreatic cancer panel for 5-FU.
Yanagihara K,Takigahira M,Tanaka H,Arao T,Aoyagi Y,Oda T,Ochiai A,Nishio K
Cancer Sci. 2008 Sep;99(9):1859-64

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LOT Information

Cell No.   JCRB1882 Cell Name   Sui65
LOT No.   02012022 Lot Specification   distribution
Medium   RPMI1640 medium(GIBCO:catalog No.11875-093) with 10% heat inactivated fetal bovine serum(Biowest cat.No.S1820-500 LotNo.S09336S1820) Temperature   37 C
Cell Density at Seeding   2.38-3.88x10^4 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.05%trypsin(GIBCO) and 0.02% EDTA.
Doubling Time   NT Cell Number in Vial (cells/1ml)   3.04x10^6
Viability at cell freezing (%)   97.2 Antibiotics Used   free
Passage Number   p4* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:11
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   BAMBANKER(LYMPHOTEC Inc.,CS-02-001,NIPPON Genetics Co., LTD) CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   97.2
Additional information