JCRB1913 Me1Tu

Cell information

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB1913 Cell Name   Me1Tu
Profile   Transplanted cell line of malignant pleural mesothelioma TCC-MESO-1 into SCID mice. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   58-year-old
Identity   not done Tissue for Primary Cancer   Pleura
Case history   Pleural epithelioid mesothelioma Metastasis   
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   high tumorigenicity, Adherent cells.
Classify   tumor Established by   Yanagihara Kazuyoshi
Registered by   Yuki Iino Regulation for Distribution   
Comment    Year   
Medium   RPMI1640 medium + 10% FBS Methods for Passages   0.05% Trypsin- 0.02% EDTA
Cell Number on Passage   split ratio=1/10 Race   Japanese
CO2 Conc.   5% Tissue Sampling   
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Reference
Pubmed id:19876922An orthotopic implantation mouse model of human malignant pleural mesothelioma for in vivo photon counting analysis and evaluation of the effect of S-1 therapy.
Yanagihara K,Tsumuraya M,Takigahira M,Mihara K,Kubo T,Ohuchi K,Seyama T
Int J Cancer. 2010 Jun 15;126(12):2835-46

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LOT Information

Cell No.   JCRB1913 Cell Name   Me1Tu
LOT No.   08182022 Lot Specification   distribution
Medium   RPMI1640 medium (GIBCO: Cat No.11875) with 10% heat inactivated fetal bovine serum (SIGMA, Cat#172012, Lot#12J396) Temperature   37 C
Cell Density at Seeding   1.3x10^4 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.05% trypsin (GIBCO) and 0.02% EDTA.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.40x10^6
Viability at cell freezing (%)   99 Antibiotics Used   free
Passage Number   p5* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:10,11
D13S317:9
D7S820:8,12
D16S539:10,11
VWA:17
TH01:7
AM:X,Y
TPOX:8,9
CSF1PO:12,14
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   BAMBANKER (LYMPHOTEC Inc., CS-02-001, NIPPON Genetics Co., LTD) CO2 Conc.   5%
Viability immediately after thawing (%)   97 Additional information   
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