JCRB1914 MEF-N7

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB1914 Cell Name   MEF-N7
Profile   Mouse Embryonic Fibroblast, derived from 12.5 day fetus, ICR, naturally immortalized cell line, can be used as a feeder Other Name   
Animal   mouse Strain   Crl:CD1(ICR)
Genus   Mus Species   musculus
Sex    Age   E12.5 fetus
Identity   not done Tissue for Primary Cancer   
Case history    Metastasis   
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   fibroblast-like Character   Mouse embryonic fibroblast derived from E12.5 fetus. A naturally immortalized cell line. This cell line can be used as a feeder.
Classify   normal Established by   Akihiro UMEZAWA
Registered by   Umezawa,A. Regulation for Distribution   
Comment    Year   
Medium   EMUKK-25 (info@emukk.com) or DMEM (high glucose) +10% FBS(HI), Can be cultivated in MEF medium. Methods for Passages   0.25% Trypsin-1mM EDTA-4Na
Cell Number on Passage    Race   
CO2 Conc.   5% Tissue Sampling   embryo/fetus
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

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Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_D5HR
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LOT Information

Cell No.   JCRB1914 Cell Name   MEF-N7
LOT No.   09092022 Lot Specification   distribution
Medium   DMEM high glucose (GIBCO Cat#11995) with 10% heat inactivated fetal bovine serum (SIGMA Cat#172012, Lot#12J396) Temperature   37 C
Cell Density at Seeding   1.2-1.8x10^4 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.25% Trypsin 1mM EDTA ･4Na (Wako #209-16941). (Split ratio=1/3)
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.45x10^6
Viability at cell freezing (%)   97 Antibiotics Used   free
Passage Number   p11 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   BAMBANKER (LYMPHOTEC Inc., CS-02-001, NIPPON Genetics Co., LTD) CO2 Conc.   5%
Viability immediately after thawing (%)   89 Additional information   
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