JCRB1919 Sui69
Cell information
Important Notice(s)Sui細胞株に関する遺伝的性質について（About the genetic properties of Sui cell lines）
Sui69の培養 - 有限増殖 / Culture of Sui69 - limited life span
Cell type：general cells (View Pricing Information)
| JCRB No. | JCRB1919 | Cell Name | Sui69 |
|---|---|---|---|
| Profile | Pancreatic adenocarcinoma. Cell line established by forming xenograft tumor (PDX) from pancreas adenocarcinoma. (The life span of this cell line is limited.) | Other Name | |
| Animal | human | Strain | |
| Genus | Homo | Species | sapiens |
| Sex | M | Age | 54-year-old |
| Identity | available | Tissue for Primary Cancer | pancreas |
| Case history | Pancreatic ductal adenocarcinoma. | Metastasis | |
| Tissue Metastasized | Genetics | Mutation of KRAS, TP53, and CDKN2A. See attached file (PDF) for details. | |
| Life Span | infinite? | Crisis PDL | |
| Morphology | epithelial-like | Character | Doubling time: 55.2 hours |
| Classify | tumor | Established by | Yanagihara Kazuyoshi |
| Registered by | Yuki Iino | Regulation for Distribution | |
| Comment | The growth of this cell line will be ceased after several passages in vitro. | Year | |
| Medium | PMI1640 medium + 10% FBS | Methods for Passages | 0.05% Trypsin- 0.02% EDTA |
| Cell Number on Passage | split ratio = 1/5 | Race | |
| CO2 Conc. | 5% | Tissue Sampling | pancreas |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | tested | Detected RNA Virus | tested | ||||||
| CMV | - | parvoB19 | - | HCV | - | HTLV-1 | - | ||
| EBV | - | HBV | - | HIV-1 | - | HTLV-2 | - | ||
| HHV6 | - | HTLV-1 | - | HIV-2 | - | HAV | - | ||
| HHV7 | - | HTLV-2 | - |
-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
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| BKV | - | HIV-1 | - | ||||||
| JCV | - | HIV-2 | - | ||||||
| ADV | - | HPV18 | - | ||||||
| Notes | |||||||||
| Reference | |
|---|---|
| Pubmed id:35272288 | A Comparative Study of Patient-Derived Tumor Models of Pancreatic Ductal Adenocarcinoma Involving Orthotopic Implantation. Yanagihara K,Iino Y,Yokozaki H,Kubo T,Oda T,Kubo T,Komatsu M,Sasaki H,Ichikawa H,Kuwata T,Seyama T,Ochiai A Pathobiology. 2022 Mar 10;():1-11 |
| Pubmed id:18691340 | Establishment and molecular profiling of a novel human pancreatic cancer panel for 5-FU. Yanagihara K,Takigahira M,Tanaka H,Arao T,Aoyagi Y,Oda T,Ochiai A,Nishio K Cancer Sci. 2008 Sep;99(9):1859-64 |
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LOT Information
| Cell No. | JCRB1919 | Cell Name | Sui69 |
|---|---|---|---|
| LOT No. | 10022023 | Lot Specification | distribution |
| Medium | RPMI1640(GIBCO Cat #11875-093) with 10-20% heat inactivated fetal bovine serum (SIGMA, Cat#172012, Lot#12J396) | Temperature | 37 C |
| Cell Density at Seeding | 1.3-1.6x10^4 cells/sq.cm | Methods for Passages | Cells were harvested after treatment with 0.05% trypsin GIBCO) and 0.02% EDTA. (Split ratio=1/3) |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 2.0x10^6 |
| Viability at cell freezing (%) | 97.80 | Antibiotics Used | free |
| Passage Number | p16 | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | D5S818:10 D13S317:11,12 D7S820:11 D16S539:10,12 VWA:16,18 TH01:6,9 AM:X,Y TPOX:8 CSF1PO:11,12 |
| Adhesion | Yes | Exoteric Gene | NT |
| Medium for Freezing | BAMBANKER (LYMPHOTEC Inc., CS-02-001, NIPPON Genetics Co., LTD) | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | 90.8 | Additional information |
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