JCRB1920 CHKS-4 Cell information
Cell type：general cells
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JCRB No.
JCRB1920
Cell Name
CHKS-4
Profile
Established from hepatocellular carcinoma in dogs. Alpha-fetoprotein producing clonal cell line.
Other Name
Animal
dog
Strain
Genus
Canis
Species
familiaris
Sex
M
Age
11-year-old
Identity
not done
Tissue for Primary Cancer
liver
Case history
Metastasis
Tissue Metastasized
Genetics
Life Span
infinite
Crisis PDL
Morphology
epithelial-like
Character
Alpha-fetoprotein producing clonal cell line.
Classify
tumor
Established by
Shinpei KAWARAI
Registered by
Shinpei KAWARAI
Regulation for Distribution
Comment
Year
2020
Medium
IMDM+10% FBS
Methods for Passages
0.1%Tripsin-EDTA
Cell Number on Passage
Race
CO2 Conc.
5%
Tissue Sampling
liver
Tissue Type
Detection of virus genome fragment by Real-time PCR
Detected DNA Virus
not tested
Detected RNA Virus
not tested
Reference
Pubmed id:16678911 Establishment and characterization of primary canine hepatocellular carcinoma cell lines producing alpha-fetoprotein.
Movies
LOT Information
Cell No.
JCRB1920
Cell Name
CHKS-4
LOT No.
10212022
Lot Specification
distribution
Medium
IMDM (GIBCO) with 10% heat inactivated fetal bovine serum (HANA-NESCO BIO:han 03041（from Dr Kawarai）)
Temperature
37 C
Cell Density at Seeding
1.3-2.5x10^4 cells/sq.cm
Methods for Passages
Cells were harvested after treatment with 0.1 % trypsin (GIBCO) and 1mM EDTA.
Doubling Time
NT
Cell Number in Vial (cells/1ml)
5.2x10^6
Viability at cell freezing (%)
99.80
Antibiotics Used
free
Passage Number
p4*
PDL
NT
Sterility: MYCOPLASMA
-
Sterility: BACTERIA
-
Sterility: FUNGI
-
Isozyme Analysis
Chromosome Mode
NT
Chromosome Information
NT
Surface Antigen
NT
DNA Profile (STR)
Adhesion
Yes
Exoteric Gene
NT
Medium for Freezing
BAMBANKER (LYMPHOTEC Inc., CS-02-001, NIPPON Genetics Co., LTD)
CO2 Conc.
5%
Viability immediately after thawing (%)
96.8
Additional information
