JCRB1922 Sui71
Cell information
Important Notice(s)Sui細胞株に関する遺伝的性質について（About the genetic properties of Sui cell lines）
Cell type：general cells (View Pricing Information)
| JCRB No. | JCRB1922 | Cell Name | Sui71 |
|---|---|---|---|
| Profile | Pancreatic adenocarcinoma. Cell line established by forming xenograft tumor (PDX) from liver metastasis. | Other Name | |
| Animal | human | Strain | |
| Genus | Homo | Species | sapiens |
| Sex | M | Age | 65-year-old |
| Identity | available | Tissue for Primary Cancer | pancreas |
| Case history | Pancreatic adenocarcinoma. Derived from metastatic site: Liver. | Metastasis | Y |
| Tissue Metastasized | liver | Genetics | Mutation of KRAS, and SMAD4. See attached file (PDF) for details. |
| Life Span | infinite | Crisis PDL | |
| Morphology | epithelial-like | Character | Doubling time: 24.1 hours |
| Classify | tumor | Established by | Yanagihara Kazuyoshi |
| Registered by | Yuki Iino | Regulation for Distribution | |
| Comment | Year | ||
| Medium | PMI1640 medium + 10% FBS | Methods for Passages | 0.05% Trypsin- 0.02% EDTA |
| Cell Number on Passage | Race | ||
| CO2 Conc. | 5% | Tissue Sampling | liver metastasis |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | tested | Detected RNA Virus | tested | ||||||
| CMV | - | parvoB19 | - | HCV | - | HTLV-1 | - | ||
| EBV | - | HBV | - | HIV-1 | - | HTLV-2 | - | ||
| HHV6 | - | HTLV-1 | - | HIV-2 | - | HAV | - | ||
| HHV7 | - | HTLV-2 | - |
-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
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| BKV | - | HIV-1 | - | ||||||
| JCV | - | HIV-2 | - | ||||||
| ADV | - | HPV18 | - | ||||||
| Notes | |||||||||
| Reference | |
|---|---|
| Pubmed id:35272288 | A Comparative Study of Patient-Derived Tumor Models of Pancreatic Ductal Adenocarcinoma Involving Orthotopic Implantation. Yanagihara K,Iino Y,Yokozaki H,Kubo T,Oda T,Kubo T,Komatsu M,Sasaki H,Ichikawa H,Kuwata T,Seyama T,Ochiai A Pathobiology. 2022 Mar 10;():1-11 |
| Pubmed id:18691340 | Establishment and molecular profiling of a novel human pancreatic cancer panel for 5-FU. Yanagihara K,Takigahira M,Tanaka H,Arao T,Aoyagi Y,Oda T,Ochiai A,Nishio K Cancer Sci. 2008 Sep;99(9):1859-64 |
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LOT Information
| Cell No. | JCRB1922 | Cell Name | Sui71 |
|---|---|---|---|
| LOT No. | 10282022 | Lot Specification | distribution |
| Medium | RPMI1640(FUJIFILM-WAKO Cat189-02025) with 10% heat inactivated fetal bovine serum (SIGMA, Cat#172012, Lot#12J396) | Temperature | 37 C |
| Cell Density at Seeding | 1.2-2.5x10x10^4 cells/sq.cm | Methods for Passages | Cells were harvested after treatment with 0.05% trypsin (GIBCO) and 0.02% EDTA (Split ratio=1/3) |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 2.62x10^6 |
| Viability at cell freezing (%) | 98.7 | Antibiotics Used | free |
| Passage Number | p7* | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | D5S818:12,13 D13S317:8,11 D7S820:8,10 D16S539:9 VWA:17 TH01:6,9 AM:X TPOX:9 CSF1PO:10,12 |
| Adhesion | Yes | Exoteric Gene | |
| Medium for Freezing | BAMBANKER (LYMPHOTEC Inc., CS-02-001, NIPPON Genetics Co., LTD) | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | 77.8 | Additional information | Start the culture with one 25cm2 flask or 60mm dish. |
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