JCRB1928 Sui76

Cell information

Important Notice(s)

Sui細胞株に関する遺伝的性質について（About the genetic properties of Sui cell lines）

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB1928 Cell Name   Sui76
Profile   Pancreatic adenocarcinoma. Cell line established by forming xenograft tumor (PDX) from liver metastasis. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex    Age   
Identity   available Tissue for Primary Cancer   pancreas
Case history   Pancreatic adenocarcinoma. Derived from metastatic site: Liver. Metastasis   Y
Tissue Metastasized   Liver Genetics   Mutation of KRAS, TP53, SMARCA4, and CDKN2A. See attached file (PDF) for details.
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   Adherent cells
Classify   tumor Established by   Yanagihara Kazuyoshi
Registered by   Yuki Iino Regulation for Distribution   
Comment    Year   
Medium   PMI1640 medium + 10% FBS Methods for Passages   0.05% Trypsin- 0.02% EDTA
Cell Number on Passage   split ratio = 1/5 Race   
CO2 Conc.   5% Tissue Sampling   liver metastasis
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Reference
Pubmed id:35272288A Comparative Study of Patient-Derived Tumor Models of Pancreatic Ductal Adenocarcinoma Involving Orthotopic Implantation.
Yanagihara K,Iino Y,Yokozaki H,Kubo T,Oda T,Kubo T,Komatsu M,Sasaki H,Ichikawa H,Kuwata T,Seyama T,Ochiai A
Pathobiology. 2022 Mar 10;():1-11

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LOT Information

Cell No.   JCRB1928 Cell Name   Sui76
LOT No.   11262022 Lot Specification   distribution
Medium   RPMI1640 medium (GIBCO：Cat No.11875) with 10% heat inactivated fetal bovine serum (SIGMA,Cat# 172012, Lot#12J396) Temperature   37 C
Cell Density at Seeding   1.36-1.90x10^4 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.05%trypsin (GIBCO) and 0.02% EDTA.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.75x10^6
Viability at cell freezing (%)   99 Antibiotics Used   free
Passage Number   p3* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:8,11
D13S317:9,12
D7S820:11,12
D16S539:9
VWA:17,19
TH01:6
AM:X,Y
TPOX:9
CSF1PO:11,12
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   BAMBANKER (LYMPHOTEC Inc., CS-02-001, NIPPON Genetics Co., LTD) CO2 Conc.   5%
Viability immediately after thawing (%)   78 Additional information   
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Cell No.   JCRB1928 Cell Name   Sui76
LOT No.   12122022 Lot Specification   distribution2
Medium   RPMI1640 medium (GIBCO：Cat No.11875) with 10% heat inactivated fetal bovine serum (SIGMA,Cat# 172012, Lot#12J396) Temperature   37 C
Cell Density at Seeding   1.48x10^4 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.05%trypsin (GIBCO) and 0.02% EDTA.
Doubling Time   NT Cell Number in Vial (cells/1ml)   8.75x10^5
Viability at cell freezing (%)   100 Antibiotics Used   free
Passage Number   p5* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:8,11
D13S317:9,12
D7S820:11,12
D16S539:9
VWA:17,19
TH01:6
AM:X,Y
TPOX:9
CSF1PO:11,12
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   BAMBANKER (LYMPHOTEC Inc., CS-02-001, NIPPON Genetics Co., LTD) CO2 Conc.   5%
Viability immediately after thawing (%)   99 Additional information   
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