JCRB1962 HT29LiLuc
Cell information
Cell type：luciferase-expressing cancer cells (View Pricing Information)
| JCRB No. | JCRB1962 | Cell Name | HT29LiLuc |
|---|---|---|---|
| Profile | Luciferase stably expressing cell line (Human Colon Cancer (HT-29) -derived liver metastasis). | Other Name | |
| Animal | human | Strain | |
| Genus | Homo | Species | sapiens |
| Sex | Age | 44-year-old | |
| Identity | available | Tissue for Primary Cancer | colon |
| Case history | Colon adenocarcinoma | Metastasis | Y |
| Tissue Metastasized | Liver | Genetics | pcDNA3.1-plus inserted with firefly luciferase gene (plasmid vector) was introduced. |
| Life Span | infinite | Crisis PDL | |
| Morphology | epithelial-like | Character | Adherent cells. |
| Classify | tumor | Established by | Yanagihara Kazuyoshi |
| Registered by | Yuki Iino | Regulation for Distribution | |
| Comment | Year | ||
| Medium | PMI1640 medium + 10% FBS + G418 400 ug/mL. Do not add G418 at thawing. | Methods for Passages | 0.05% Trypsin- 0.02% EDTA |
| Cell Number on Passage | Race | ||
| CO2 Conc. | 5% | Tissue Sampling | colon |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | tested | Detected RNA Virus | tested | ||||||
| CMV | - | parvoB19 | - | HCV | - | HTLV-1 | - | ||
| EBV | - | HBV | - | HIV-1 | - | HTLV-2 | - | ||
| HHV6 | - | HTLV-1 | - | HIV-2 | - | HAV | - | ||
| HHV7 | - | HTLV-2 | - |
-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
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| BKV | - | HIV-1 | - | ||||||
| JCV | - | HIV-2 | - | ||||||
| ADV | - | HPV18 | - | ||||||
| Notes | |||||||||
| Reference | |
|---|---|
| Pubmed id:30560565 | Antitumor effect of palmitic acid-conjugated DsiRNA for colon cancer in a mouse subcutaneous tumor model. Kubo T,Nishimura Y,Hatori Y,Akagi R,Mihara K,Yanagihara K,Seyama T Chem Biol Drug Des. 2019 Apr;93(4):570-581 |
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LOT Information
| Cell No. | JCRB1962 | Cell Name | HT29LiLuc |
|---|---|---|---|
| LOT No. | 08142023 | Lot Specification | distribution |
| Medium | RPMI1640(GIBCO Cat #11875-093) with 10% heat inactivated fetal bovine serum (SIGMA, Cat#172012, Lot#12J396) and G418(400 ug/ml GIBCO REF 10131-035). Do not add G418 at thawing. | Temperature | 37 C |
| Cell Density at Seeding | 0.45-5.86x10^5 cells/sq.cm | Methods for Passages | Cells were harvested after treatment with 0.05% trypsin (GIBCO) and 0.02% EDTA. |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 6.56x10^6 |
| Viability at cell freezing (%) | 80.68 | Antibiotics Used | G418 |
| Passage Number | p5* | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | D5S818:11,12 D13S317:11,12 D7S820:10 D16S539:11,12 VWA:17,19 TH01:6,9 AM:X TPOX:8,9 CSF1PO:11,12 |
| Adhesion | Yes | Exoteric Gene | NT |
| Medium for Freezing | BAMBANKER (LYMPHOTEC Inc., CS-02-001, NIPPON Genetics Co., LTD) | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | 77.91 | Additional information | Do not add G418 at thawing. |
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