JCRB1972 Kit225
Cell information
Important Notice(s)Kit225細胞の分譲について：営利機関は細胞樹立者とのMTA締結が必要です
Cell type：general cells (View Pricing Information)
| JCRB No. | JCRB1972 | Cell Name | Kit225 |
|---|---|---|---|
| Profile | IL-2 dependent T-cell chronic lymphocytic leukemia (T-CLL) cell line | Other Name | |
| Animal | human | Strain | |
| Genus | Homo | Species | sapiens |
| Sex | M | Age | 62-year-old |
| Identity | available | Tissue for Primary Cancer | |
| Case history | T-cell chronic lymphocytic leukemia | Metastasis | |
| Tissue Metastasized | Genetics | ||
| Life Span | Crisis PDL | ||
| Morphology | lymphocyte-like | Character | |
| Classify | tumor | Established by | Toshiyuki HORI |
| Registered by | Toshiyuki HORI | Regulation for Distribution | Profit organization should conclude MTA with the developer. |
| Comment | Year | ||
| Medium | RPMI1640 medium + 10%FBS with 1 U/mL human IL-2 | Methods for Passages | dilution |
| Cell Number on Passage | Race | ||
| CO2 Conc. | 5% | Tissue Sampling | perpheral blood |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | tested | Detected RNA Virus | not tested | ||||||
| CMV | - | parvoB19 | - |
-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
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| EBV | - | HBV | - | ||||||
| HHV6 | - | HTLV-1 | - | ||||||
| HHV7 | - | HTLV-2 | - | ||||||
| BKV | - | HIV-1 | - | ||||||
| JCV | - | HIV-2 | - | ||||||
| ADV | - | HPV18 | - | ||||||
| Notes | |||||||||
| Reference | |
|---|---|
| Pubmed id:3115332 | Establishment of an interleukin 2-dependent human T cell line from a patient with T cell chronic lymphocytic leukemia who is not infected with human T cell leukemia/lymphoma virus. Hori T,Uchiyama T,Tsudo M,Umadome H,Ohno H,Fukuhara S,Kita K,Uchino H Blood. 1987 Oct;70(4):1069-72 |
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LOT Information
| Cell No. | JCRB1972 | Cell Name | Kit225 |
|---|---|---|---|
| LOT No. | 09152023 | Lot Specification | distribution |
| Medium | RPMI1640(GIBCO Cat #11875-093) with 10% heat inactivated fetal bovine serum (SIGMA, Cat#172012, Lot#12J396) and 1 U/ml rHu IL-2 (10 U/ml until growing well) | Temperature | 37 C |
| Cell Density at Seeding | 1-2.5x10^5 cells/ml | Methods for Passages | Dilution (1/3-1/9) |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 1.68x10^6 |
| Viability at cell freezing (%) | 89.6 | Antibiotics Used | free |
| Passage Number | p5* | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | D5S818:10,12 D13S317:9,12 D7S820:8,11 D16S539:9,12 VWA:17,19 TH01:7,9 AM:X,Y TPOX:8,11 CSF1PO:10,12 |
| Adhesion | No | Exoteric Gene | NT |
| Medium for Freezing | BAMBANKER (LYMPHOTEC Inc., CS-02-001, NIPPON Genetics Co., LTD) | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | 78.2 | Additional information | It is recomended to be 10 U/ml IL-2 until growing well. |
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