JCRB1981 BT-J

Cell information

Important Notice(s)

BT細胞系の培養について

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB1981 Cell Name   BT-J
Profile   bovine trophoblastic cell line Other Name   
Animal   bovine Strain   
Genus   Bos Species   taurus
Sex    Age   
Identity   not done Tissue for Primary Cancer   
Case history    Metastasis   
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   
Classify   normal Established by   Hashizume K., Takahashi T.
Registered by   Keiichiro Kizaki Regulation for Distribution   
Comment   BMP4 concentration at cell establishment: 100 ng/mL Year   
Medium   DMEM/F12 medium + 10% FBS (Culture in 20% FBS when thawing), Use collagen-coated dish or flask (Cellmatrix Type I-C, Nitta Gelatin, Osaka,Japan) Methods for Passages   Pipetting dissociation and dilution.
Cell Number on Passage    Race   
CO2 Conc.   5% Tissue Sampling   blastcyst
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Reference
Pubmed id:21862694Bone morphogenetic protein 4 accelerates the establishment of bovine trophoblastic cell lines.
Suzuki Y,Koshi K,Imai K,Takahashi T,Kizaki K,Hashizume K
Reproduction. 2011 Nov;142(5):733-43

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LOT Information

Cell No.   JCRB1981 Cell Name   BT-J
LOT No.   11022023 Lot Specification   distribution
Medium   DMEM/ F12 medium (SIGMA, #D8062-500ML) or DMEM/F-12, GlutaMAX™ supplement (GIBCO, #10565-018) with10% (20%) heat inactivated fetal bovine serum (JAPAN・BIOSERUM, #NZP 001, Lot CNGGR-100). Using collagen type I coated flask (CORNING, #NCO430639) Temperature   37 C
Cell Density at Seeding   uncountable Methods for Passages   Pipetting dissociation and dilution. (split ratio=1/3-1/4)
Doubling Time   NT Cell Number in Vial (cells/1ml)   uncountable
Viability at cell freezing (%)   Uncountable Antibiotics Used   free
Passage Number   p49 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   BAMBANKER (NIPPON Genetics Co., LTD, #CS-02-001) CO2 Conc.   5%
Viability immediately after thawing (%)   uncountable Additional information   We recommend to culture initially with 20% heat inactivated fetal bovine serum.
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