JCRB4016 WILL3
Cell information
Important Notice(s)Resuscitation of WILL3 cells / WILL3細胞の解凍と培養法
Cell type：general cells (View Pricing Information)
| JCRB No. | JCRB4016 | Cell Name | WILL3 |
|---|---|---|---|
| Profile | Human diffuse large B-cell lymphoma cell line with two translocations t(14;18)(q32; q21) and t(8;22)(q24;q11). | Other Name | |
| Animal | human | Strain | |
| Genus | Homo | Species | sapiens |
| Sex | F | Age | 60-year-old |
| Identity | available | Tissue for Primary Cancer | bone marrow |
| Case history | diffuse large B-cell lymphoma：DLBCL | Metastasis | |
| Tissue Metastasized | Genetics | t(14;18)(q32; q21) and t(8;22)(q24;q11) | |
| Life Span | infinite | Crisis PDL | |
| Morphology | lymphocyte-like | Character | WILL3 lacks surface IGs and produces no IG protein. |
| Classify | tumor | Established by | Takasi SONOKI |
| Registered by | Sonoki,T. | Regulation for Distribution | |
| Comment | Year | ||
| Medium | RPMI1640 medium with 10% FBS | Methods for Passages | dilution |
| Cell Number on Passage | Race | Japanese | |
| CO2 Conc. | 5% | Tissue Sampling | bone marrow |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | tested | Detected RNA Virus | not tested | ||||||
| CMV | - | parvoB19 | - |
-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
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| EBV | - | HBV | - | ||||||
| HHV6 | - | HTLV-1 | - | ||||||
| HHV7 | - | HTLV-2 | - | ||||||
| BKV | - | HIV-1 | - | ||||||
| JCV | - | HIV-2 | - | ||||||
| ADV | - | HPV18 | - | ||||||
| Notes | |||||||||
| Reference | |
|---|---|
| Pubmed id:none | IGLL5 controlled by super-enhancer affects cell survival and MYC expression in mature B-cell lymphoma Hiroki Hosoi, Shotaro Tabata, Hideki Kosako, Yoshikazu Hori, Tadashi Okamura, Yusuke Yamashita, Kota Fujimoto, Daiki Kajioka, Kentaro Suzuki, Motomi Osato, Gen Yamada, Takashi Sonoki, Leukemia Research Reports 21 (2024) 100451 |
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LOT Information
| Cell No. | JCRB4016 | Cell Name | WILL3 |
|---|---|---|---|
| LOT No. | 04012024 | Lot Specification | distribution |
| Medium | RPMI1640(GIBCO Cat #11875-093) with 10% heat inactivated fetal bovine serum (SIGMA, Cat#172012, Lot#12J396) | Temperature | 37 C |
| Cell Density at Seeding | 1.69-5.23x10^5 cells/mL | Methods for Passages | dilution |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 5.99x10^6 |
| Viability at cell freezing (%) | 99 | Antibiotics Used | free |
| Passage Number | p4* | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | D5S818:9,12 D13S317:10,11 D7S820:11 D16S539:10,11 VWA:15,17 TH01:6,9 AM:X TPOX:11 CSF1PO:12,13 |
| Adhesion | No | Exoteric Gene | NT |
| Medium for Freezing | BAMBANKER (LYMPHOTEC Inc., CS-02-001, NIPPON Genetics Co., LTD) | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | 99 | Additional information |
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