JCRB4026 KE-66
Cell information
Cell type：general cells (View Pricing Information)
| JCRB No. | JCRB4026 | Cell Name | KE-66 |
|---|---|---|---|
| Profile | Poorly differentiated adenocarcinoma with giant cells | Other Name | |
| Animal | human | Strain | |
| Genus | Species | ||
| Sex | F | Age | 79-year-old |
| Identity | available | Tissue for Primary Cancer | pancreas |
| Case history | ly3, v3, pn3, du2 | Metastasis | Y |
| Tissue Metastasized | Genetics | KRAS G12D homo | |
| Life Span | infinite | Crisis PDL | |
| Morphology | epithelial-like | Character | DT=48 hrs |
| Classify | tumor | Established by | Kazuya YAMASHITA |
| Registered by | YAMASHITA Kazuya | Regulation for Distribution | |
| Comment | Year | ||
| Medium | MEM+10%FBS | Methods for Passages | PBS(-) wash 2 times (5min), 0.25%Trypsin+0.02%EDTA, passage 1:10split /1 week, Saturation density 2.6 x 10^5 cells/cm^2 |
| Cell Number on Passage | 2.5 x 10^5 cells/25cm^2 | Race | |
| CO2 Conc. | 5% | Tissue Sampling | primary pancreas carcinoma |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | not tested | Detected RNA Virus | not tested | ||||||
| Reference | |
|---|---|
| Pubmed id:none | 巨細胞を伴う膵癌由来細胞株の樹立と性状. 山下和也, 他 第11回 ヒト細胞学会抄録 1993. 8. 27. |
| Pubmed id:none | 細胞株を用いた免疫組織化学染色マルチプルコントロール試料の作製と標準化の基礎的検討. 山下和也, 他 神奈川県臨床検査技師会雑誌(2188-6628) |
LOT Information
| Cell No. | JCRB4026 | Cell Name | KE-66 |
|---|---|---|---|
| LOT No. | 06182024 | Lot Specification | distribution |
| Medium | MEM (GIBCO cat.No.11095-080) with 10% fetal bovine serum (Nichirei Lot No.20M00K) | Temperature | 37 C |
| Cell Density at Seeding | 2.0-5.3x10^4 cells/sq.cm | Methods for Passages | Cells were harvested after treatment with 0.25%trypsin (GIBCO) and 0.02% EDTA. (Split ratio=1/10 once a week) |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 2.8x10^6 |
| Viability at cell freezing (%) | 99.60 | Antibiotics Used | free |
| Passage Number | p6* | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | D5S818:11 D13S317:12 D7S820:8,12 D16S539:10,13 VWA:17 TH01:6,7 AM:X TPOX:8 CSF1PO:10 |
| Adhesion | Exoteric Gene | ||
| Medium for Freezing | BAMBANKER (LYMPHOTEC Inc., CS-02-001, NIPPON Genetics Co., LTD) | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | 78.5 | Additional information | We recommend to start culture with 25T Flask, cause of low adhesion rate. |