JCRB4035 Daudi-
Cell information
Cell type：general cells (View Pricing Information)
| JCRB No. | JCRB4035 | Cell Name | Daudi- |
|---|---|---|---|
| Profile | A clone in which EBV was dropped from Daudi+ | Other Name | |
| Animal | human | Strain | |
| Genus | Homo | Species | sapiens |
| Sex | M | Age | 16-year-old |
| Identity | available | Tissue for Primary Cancer | blood |
| Case history | Epstein-Barr virus-related Burkitt lymphoma | Metastasis | |
| Tissue Metastasized | Genetics | ||
| Life Span | infinite | Crisis PDL | |
| Morphology | lymphocyte-like | Character | |
| Classify | tumor | Established by | Kenzo Takada |
| Registered by | Kenzo Takada | Regulation for Distribution | |
| Comment | Year | 2019 | |
| Medium | RPMI1640 medium with 20% FBS. | Methods for Passages | dilution. As cell density increases, cells die immediately. |
| Cell Number on Passage | Race | ||
| CO2 Conc. | 5% | Tissue Sampling | bood |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | tested | Detected RNA Virus | tested | ||||||
| CMV | - | parvoB19 | - | HCV | - | HTLV-1 | - | ||
| EBV | - | HBV | - | HIV-1 | - | HTLV-2 | - | ||
| HHV6 | - | HTLV-1 | - | HIV-2 | - | HAV | - | ||
| HHV7 | - | HTLV-2 | - |
-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
|||||
| BKV | - | HIV-1 | - | ||||||
| JCV | - | HIV-2 | - | ||||||
| ADV | - | HPV18 | - | ||||||
| Notes | |||||||||
| Reference | |
|---|---|
| Pubmed id:11867523 | Epstein-Barr virus RNA confers resistance to interferon-alpha-induced apoptosis in Burkitt's lymphoma. Nanbo A,Inoue K,Adachi-Takasawa K,Takada K EMBO J. 2002 Mar 1;21(5):954-65 |
LOT Information
| Cell No. | JCRB4035 | Cell Name | Daudi- |
|---|---|---|---|
| LOT No. | 09172024 | Lot Specification | distribution |
| Medium | RPMI1640 medium (GIBCO:catalog No.11875) with 20% heat inactivated fetal bovine serum (Nichirei Cat. # 174112, Lot 20M00K) | Temperature | 37 C |
| Cell Density at Seeding | 1.6-8.8x10^4 cells/ml | Methods for Passages | Dilution (split ratio=1/10-20) twice per week. |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 2.0x10^6 |
| Viability at cell freezing (%) | 98.20 | Antibiotics Used | free |
| Passage Number | p13* | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | NT |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | D5S818:8,13 D13S317:11,12 D7S820:8,10 D16S539:10,12 VWA:15,17 TH01:6,7 AM:X,Y TPOX:8,11 CSF1PO:12 |
| Adhesion | No | Exoteric Gene | NT |
| Medium for Freezing | BAMBANKER (LYMPHOTEC Inc., CS-02-001, NIPPON Genetics Co., LTD) | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | 79.7 | Additional information | This cell line was found to be contaminated by mycoplasmas at the deposition of cell bank. The mycoplasma was eliminated by the treatment with plasmocin by JCRB Cell Bank. The current lots for distribution are mycoplasma-free. |