JCRB4052 Daudi+

Cell information

Important Notice(s)

Limited to academic research use only / 学術研究目的のみの利用に限定 (JCRB4052 Daudi+)

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB4052 Cell Name   Daudi+
Profile   lymphoma, Burkitt's, Deposit from the same scientist as Daudi- depositories. Other Name   Daudi
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   16-year-old
Identity   available Tissue for Primary Cancer   blood
Case history   Epstein-Barr virus-related Burkitt lymphoma Metastasis   
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   lymphocyte-like Character   
Classify   tumor Established by   Klein E
Registered by   Kenzo Takada Regulation for Distribution   Academic research only
Comment    Year   2019
Medium   RPMI1640 medium with 20% FBS. Methods for Passages   dilution. As cell density increases, cells die immediately.
Cell Number on Passage    Race   
CO2 Conc.   5% Tissue Sampling   blood
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV + HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Reference
Pubmed id:11867523Epstein-Barr virus RNA confers resistance to interferon-alpha-induced apoptosis in Burkitt's lymphoma.
Nanbo A,Inoue K,Adachi-Takasawa K,Takada K
EMBO J. 2002 Mar 1;21(5):954-65
Pubmed id:4174339Surface IgM-kappa specificity on a Burkitt lymphoma cell in vivo and in derived culture lines.
Klein E,Klein G,Nadkarni JS,Nadkarni JJ,Wigzell H,Clifford P
Cancer Res. 1968 Jul;28(7):1300-10




LOT Information

Cell No.   JCRB4052 Cell Name   Daudi+
LOT No.   11152024 Lot Specification   distribution
Medium   RPMI1640 medium (GIBCO:catalog No.11875) with 20% heat inactivated fetal bovine serum (Nichirei Cat. # 174112, Lot 20M00K) Temperature   37 C
Cell Density at Seeding   3.1x10^4 cells/ml Methods for Passages   Dilution (split ratio=1/10-20) twice per week.
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.5x10^6
Viability at cell freezing (%)   98.20 Antibiotics Used   free
Passage Number   p5* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:8,13
D13S317:11,12
D7S820:8,10
D16S539:10,12
VWA:15,17
TH01:6,7
AM:X,Y
TPOX:8,11
CSF1PO:12
Adhesion   No Exoteric Gene   NT
Medium for Freezing   BAMBANKER (LYMPHOTEC Inc., CS-02-001, NIPPON Genetics Co., LTD) CO2 Conc.   5%
Viability immediately after thawing (%)   86.3 Additional information   Becouse the cells are so small,we reccomend centrifugation at 1,200 rpm for 10 miniutes.
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