JCRB1355 1-1ras1000

Cell information

Important Notice(s)

Balb/c 3T3 A31-1に派生する細胞株の由来の問題について

The problem of origin of Balb/c 3T3 A31-1 derived cell lines.

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB1355 Cell Name   1-1ras1000
Profile   The ras-transformed BALB/c 3T3 A31-1-1variant cell lines were strongly tumorinenic, but the cells were not metastatic. (Strain problem was found. Refer Important Notice at cell detail page.) Other Name   
Animal   mouse Strain   Swiss
Genus   Mus Species   musclus
Sex    Age   embryo
Identity    Tissue for Primary Cancer   embryo
Case history   normal Metastasis   No
Tissue Metastasized    Genetics   Origin of this cell line, Balb/c 3T3 A31-1, was found not to be derived from BALB 3T3 A31.
Life Span   infinite Crisis PDL   
Morphology   fibroblast-like Character   Fibroblast, ras-transformed subclone of Balb/3T3 A31-1-1.
Classify   transformed Established by   Tatsuka, M.
Registered by   Ota, T. Regulation for Distribution   
Comment    Year   2008
Medium   Eagle's minimal essential medium with 10% fetal calf serum Methods for Passages   0.25% trypsin & 0.02% EDTA. Practically, 1/100 dilution once a week. Avoid to be confluent.
Cell Number on Passage   --- Race   
CO2 Conc.   5% Tissue Sampling   embryo, whole
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_8653
Pubmed id:9096670A BALB/c 3T3-transformed cell line suitable for transfection assay of metastasis-inducing genes.
Tatsuka M,Ota T,Maeda M,Wada M,Yamagishi N,Taniguchi S,Seiki M,Odashima S
Int J Cancer. 1997 Mar 28;71(1):88-93
Pubmed id:7394516Cell variants showing differential susceptibility to ultraviolet light--induced transformation.
Kakunaga T,Crow JD
Science. 1980 Jul 25;209(4455):505-7
Pubmed id:4311790Murine sarcoma and leukemia viruses: assay using clonal lines of contact-inhibited mouse cells.
Jainchill JL,Aaronson SA,Todaro GJ
J Virol. 1969 Nov;4(5):549-53
Pubmed id:4301006Development of 3T3-like lines from Balb-c mouse embryo cultures: transformation susceptibility to SV40.
Aaronson SA,Todaro GJ
J Cell Physiol. 1968 Oct;72(2):141-8
Pubmed id:13985244Quantitative studies of the growth of mouse embryo cells in culture and their development into established lines.
J Cell Biol. 1963 May;17():299-313


LOT Information

Cell No.   JCRB1355 Cell Name   1-1ras1000
LOT No.   11252009 Lot Specification   distribution
Medium   Eagle's minimum essential medium(Nissui) with 10% heat inactivated fetal bovine serum(SIGMA027K03911) Temperature   37 C
Cell Density at Seeding   0.5-5x10^3 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA.1/100 split once a week.Avoid to be confluent.
Doubling Time   NT Cell Number in Vial (cells/1ml)   4.51x10^6
Viability at cell freezing (%)   99.4 Antibiotics Used   free
Passage Number   p3* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information