1. What need to do when cell lines arrive?

  1. Frozen cells are cryopreserved in a vial and will be dispatched by courier in dry ice.
  2. It is recommended to start cultivation upon receipt of the cells, according to the optimum conditions for growth of that cell.
  3. If you with to store frozen vials, we ask you to transfer vials to the vapor phase of liquid nitrogen immediately after arrival. Do not immerse the vials in the liquid phase, as this could cause them to burst.
  4. Cells can be stored in a low temperature freezer at below -80°C for short-term storage of up to 30 days. Do not store them at -30°C, as this results in a rapid decrease in viability.

2. Can I store cell vials in liquid nitrogen?

Do not store vials in liquid nitrogen directly. If vials are immersed directly in liquid nitrogen (liquid phase), it may cause explosion of imperfectly sealed vials. It is recommended to reserve these vials in the vapor phase of liquid nitrogen tank.    

3. How do I start culturing and safely open glass ampules?

  1. Remove a vial from the package using safety gloves and a face shield to avoid injury from possible explosion.
  2. Thaw the vial(s) one by one within 2 min by shaking in moderately warm water (below 37°C).
  3. Under aseptic conditions, sterilize the entire surface of the vial with gauze moistened with 70% ethanol or cationic detergent sterilizer, then open the vial. If the vial is glass ampoule, cut the neck off while wrapped in sterilized gauze. All glass ampules are pre-scored around the neck to be easily cut off, but please take extra care to avoid injury.
  4. Transfer the cell suspension to a centrifuge tube, add 10 ml of the medium described in the cell data, centrifuge the mixture at 1000 rpm for 3 min, and discard the supernatant.
  5. Re-suspend the cells in the medium. Culture them in a flask or a dish using the standard method. The appropriate culture volume would be 5 ml per vial in a 25cm2 flask or 6cm dish, however, the volume of dishes must be flexible according to actual cell density. Make sure of cell proliferation before proceeding to passage, because excessive dilution may lead to cell death, especially in hemocytes and lymphocytes.
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