1. Purchase method and form procedure

  2. Just after receiving cells

    1. What do I need to do when cell lines arrive?
    2. Can I store vials in liquid nitrogen?
    3. How do I start culturing? How do I safely open glass vials?
  3. Medium

    1. Important notes about media
    2. May I use my own medium instead of medium specified in the data sheet?
    3. Glucose concentration for DMEM medium
  4. Successful cell culture

    1. Cell culture process
    2. Guidelines for maintaining CO2 incubator
    3. Dishes and flasks
  5. Troubleshooting

    1. Recovery from proliferation failure of human lymphocytic cells
    2. Contact information for technical inquiries