JCRB0744 ECV304
細胞情報
Important Notice(s)Cross-contamination between ECV304 and T24
細胞種類：一般細胞 (細胞分譲手数料はこちら)
| 細胞番号(JCRB) | JCRB0744 | 細胞名 | ECV304 |
|---|---|---|---|
| 生物種(日本語) | ヒト | 組織名(日本語) | 血管系 |
| コメント(日本語) | 品質管理上の問題が生じたため分譲停止（詳細はお問い合わせください）。不死化正常血管内皮細胞として樹立, | プロフィール | Stop providing for the issue of quality control. (Please contact us for more information) Originally this cell line was established as human endothelium cells from umbilical cord but later the cell line was identified to be an EJ1/T24 by STR-PCR analysis |
| 別名 | 動物名 | human | |
| 系統名 | 学名・属名 | Homo | |
| 種名 | sapiens | 性別 | M |
| 年齢・月齢 | 0-years-old | 細胞識別情報 | available |
| （癌）原発組織名 | vascular system | 病歴情報 | normal |
| 転移の有無（Y/N） | No | （癌）転移組織名 | |
| 遺伝的性質 | human immortalized endothelium cell | 細胞寿命 | infinite |
| ｸﾗｲｼｽＰＤＬ | 形態 | epithelial-like | |
| 一般性状 | human immortalized endothelium cell line from umbilical cord. But JCRB identified the cell line is derived from cross contamination with famous EJ1/T24 cells by STR-PCR analysis. | 細胞分類 | transformed |
| 細胞樹立者名 | Takahashi,K. | 細胞寄託者 | Umeda,M. |
| 分譲時制限 | Refer Ref.{3581} when publish paper using the cell line ECV304. | コメント | Dr. Drexler of the DSM informed to us that the ECV304 was crosscontaminated with EJ1/T24 at the end of 1998. Following his suggestion the JCRB examined by STR-PCR method and confirmed the crosscontamination. |
| 入手年 | 1991 | 培養培地 | M199 medium with 10% fetal bovine serum. |
| 継代方法 | Cells are hrvested after treatment with 0.125% trypsin and 0.02% EDTA. | 継代時細胞数 | 7.3 x 10^3 cells/sq.cm. |
| 人種 | 炭酸ガス濃度 | 5 % | |
| 採取組織名 | umbilical cord | 組織型 |
| ウイルスDNA・RNA検出検査 (Detection of virus genome fragment by Real-time PCR) | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| ウイルスDNA 検出検査 |
tested | ウイルスRNA 検出検査 |
not tested | ||||||
| CMV | - | parvoB19 | - |
-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
|||||
| EBV | - | HBV | - | ||||||
| HHV6 | - | HTLV-1 | - | ||||||
| HHV7 | - | HTLV-2 | - | ||||||
| BKV | - | HIV-1 | - | ||||||
| JCV | - | HIV-2 | - | ||||||
| ADV | - | HPV18 | - | ||||||
| Notes | |||||||||
| Reference | |
|---|---|
| Pubmed id:1378828 | Rare spontaneously transformed human endothelial cell line provides useful research tool. Takahasi K,Sawasaki Y In Vitro Cell Dev Biol. 1992 Jun;28A(6):380-2 |
| Pubmed id:1690702 | Spontaneous transformation and immortalization of human endothelial cells. Takahashi K,Sawasaki Y,Hata J,Mukai K,Goto T In Vitro Cell Dev Biol. 1990 Mar;26(3 Pt 1):265-74 |
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